
The GRT Hub provides the following services for checking the quality of DNA and RNA samples.
Please aliquot at least 5ul of sample for up to 2 QC services.
Nanodrop - Core service
- Nanodrop quantification is commonly used to quantify DNA or RNA using UV absorbance.
- Instrument: Nanodrop 1000
Qubit Quantification – Core Service
Qubit quantification uses fluorescence dyes that specifically bind to DNA or RNA. Qubit is typically more accurate than quantification by UV absorbance.
Instrument: Qubit 3
Qubit assays offered at GRT Hub:
- dsDNA High Sensitivity (0.2-100ng)
- dsDNA Broad (2-1000ng)
- RNA High Sensitivity (5-100ng).
Agilent Bioanalyzer – Core Service
The bioanalyzer is a small-scale gel electrophoresis system, using a micro-capillary electrophoretic chip.
Instrument: Agilent 2100 Bioanalyzer
Bioanalyzer assays available at GRT Hub:
- RNA
- RNA Pico (0.1-5 ng/uL)
- RNA Nano (25-500ng/uL)
- Small RNA
- DNA
- DNA
- High Sensitivity (5-500 pg/uL of DNA fragments between 50-7000bp)
- DNA 12000 (0.5-50 ng/uL of DNA fragments between 100-12000bp)
Kapa qPCR Quantification – Both Walk-up and Core Service
- Kapa qPCR is used to quantify Illumina compatible libraries. Accurate quantification of bona fide PCR-competent sequencing templates is crucial for reliable clonal amplification via bridge PCR (bPCR; “cluster amplification”).
- Instruments: BioRad CFX96, QuantStudio Flex 7
AMPure XP Bead Clean Up – Core Service
- AMPure XP beads use a multifactorial equilibrium chemistry for binding, washing, and elution to allow for removal of contaminants and undesirable DNA fragments such as adapter dimers.
Corvaris Shearing comment on fragment sizes? – Both Walk-up and Core Service
- The Adaptive Focused Acoustics® (AFA®) process is conducted under isothermal conditions to shear DNA and RNA without GC bias or thermal damage.
- Instrument: Corvaris S220, S-Series Holder microtube-50 Screw-Cap (PN 500492)
- Consumables available at the core for a fee: microtube-50 AFA Fiber Screw-Cap (PN 520166)
RNA Purification + DNase I Treatment – Core Service
- For RNA samples with genomic DNA contamination, RNA purification which includes a DNase I treatment can be performed.
Speed Vacuum Concentrator – Core Service
- Thermo Scientific Savant SpeedVac vacuum concentrators will remove solvents and concentrate samples while maintaining sample integrity.