The GRT Hub has systems for quantitative PCR (qPCR) using the BioRad qPCR real time PCR system for quantitative comparison among samples and also for absolute measurements using digital PCR with the BioRad ZX200 ddPCR or the ThermoFisher Quantstudio Q which can measure up to three targets simultaneously.
Using the Bio-Rad’s QX200 Droplet Digital PCR (ddPCR) System you can perform high-throughput digital PCR without using as much sample and reagents as you would with other means. Droplet digital PCR is essentially when the DNA is partitioned into nanoliter sized droplets, where PCR amplification occurs (learn more on the BioRad website). The GRT Hub also has the ThermoFisher Quantstudio Q for digital PCR analysis. The Quantstudio Q can assay up to 16 samples, uses less reagents and is a faster workflow in comparison to the Bio-Rad system. Both platforms are available as a walk-up service.