Library Prep

Request Service via iLab (have this as a hyperlink button to iLab: https://uci.ilab.agilent.com/service_center/show_external/5672)

RNA - Sequencing

GRT Hub offers several library construction options depending on the source and quality of the RNA sample:

Poly-A Enrichment Library Construction

This type of library construction will enrich for mRNA by hybridizing the poly A-tail on mRNA with the poly-T oligos on magnetic beads. This method requires high quality total RNA, with a RIN score greater than 8.

rRNA Depletion Library Construction

This type of library construction removes ribosomal RNA from the total RNA sample. This method will enrich for non-ribosomal poly-adenylated and non-polyadenylated RNA.  The rRNA depletion workflow can be used with degraded RNA.

Micro RNA and Small RNA Library Construction

GRT Hub also offers Micro RNA and Small RNA library construction from total RNA. However, please ensure that the RNA isolation method retains micro and small RNA.

 

Please contact GRT Hub if you have any questions about RNA-sequencing.

 

DNA - Sequencing

GRT Hub offers several library construction options for DNA sequencing.

 

Whole Genome Sequencing

 

Exome Sequencing

Targeted Amplicon

Microbiome (16S/18S/ITS)

ATAC-Seq