One-Day Visium HD Spatial Gene Expression Data Analysis Workshop

10x Genomics

One-Day Visium HD Spatial Gene Expression Data Analysis Workshop

Wednesday 06/25/2025

9:00 AM to 4:00 PM - Lunch is provided - Registration is Required

Medical Education Building, Room 4020

In this hands-on workshop, you’ll embark on the Visium HD Spatial Gene Expression data analysis journey with us. Using example human colon cancer sample data , we’ll begin by processing raw data with Space Ranger on the 10x Cloud Analysis platform. Next, we’ll assess data quality using the web summary and then dive into Loupe Browser for in-depth data exploration and cell-type mapping. We’ll then take these insights to community-developed tools, further exploring the tumor microenvironment through pathway analysis. To conclude, each participant will have the opportunity to discuss their own analysis roadmap with guidance from 10x Genomics experts. Even though this workshop is focused on Visium HD Spatial Gene data, parts of the workshop will also be useful for single cell experiments

The event is free, but registration is required.

Agenda:
● Introduction
● Overview of Visium HD spatial data analysis
● 10x Cloud Analysis: from FASTQs to quantitative biological information with Space Ranger for
Visium HD
● Quality assessment: interpreting the Space Ranger web summary
● Loupe Browser: data exploration and cell-type mapping
● Introducing community-developed tools for Visium data analysis
● Further analysis using community tools: GSEA as an example
● Wrap up and optional chat with the 10x team